MAKING THE SMEAR
Place a small drop of blood near an end of a slide. According to figure 7, bring the edge of another slide in contact with the drop and allow the drop to bank evenly behind the spreader. The angle between the two slides has to be 30-40 degrees. Now, push to the left in a smooth, quick motion. The smear should cover about half the slide. It is important that the quantity of blood is not excessive, otherwise the red cells could hide the leukocytes. So, if you succeed in making a gradual transition from thick to thin in your smear, you should get a zone with a satisfactory distribution of cells.With a single drop of blood, you can make several smears. In fact, to make a smear, it is enough to leave a spot of blood of 3 mm about in diameter on the slide. It is useful to perform many smears. In fact, not always they are successful, and with some attempts, it is easier to get one well prepared. To avoid producing clots, you must make each smear with fresh blood and straight after having deposited it. To this purpose, it is useful to be helped by another person where one deposits the blood, and the other makes the smears. With the microscope, you should observe the smears to check that some of them are properly made. The red cells must not overlap each other, nor be so scarce as to be too spread out.
Place a small drop of blood near an end of a slide. According to figure 7, bring the edge of another slide in contact with the drop and allow the drop to bank evenly behind the spreader. The angle between the two slides has to be 30-40 degrees. Now, push to the left in a smooth, quick motion. The smear should cover about half the slide. It is important that the quantity of blood is not excessive, otherwise the red cells could hide the leukocytes. So, if you succeed in making a gradual transition from thick to thin in your smear, you should get a zone with a satisfactory distribution of cells.With a single drop of blood, you can make several smears. In fact, to make a smear, it is enough to leave a spot of blood of 3 mm about in diameter on the slide. It is useful to perform many smears. In fact, not always they are successful, and with some attempts, it is easier to get one well prepared. To avoid producing clots, you must make each smear with fresh blood and straight after having deposited it. To this purpose, it is useful to be helped by another person where one deposits the blood, and the other makes the smears. With the microscope, you should observe the smears to check that some of them are properly made. The red cells must not overlap each other, nor be so scarce as to be too spread out.
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